In tissue engineering (TE), physical stimuli, such as stretch or electrical pulses, have been demonstrated to be crucial for supporting several biological processes, e.g. for promoting the cell differentiation or the tissue maturation. Bioreactors, designed for providing in vitro physical stimuli, represent powerful tools to elucidate defined cellular mechanotransduction pathways. Indeed, they allow for investigating the relationship between in vivo-like physiological or pathological chemical and physical cues and cell response by enabling the setting of closely controlled and monitored 3D dynamic conditions, hence overcoming the limits of static cell cultures as well as ensuring standardization of the processes. In particular, bioreactors for providing mechanical stimulations are usually composed of a culture chamber, an actuation unit, and a control unit.