Chronic wounds (CWs) are a leading public health concern worldwide representing an economic and social burden and affecting patient’s quality of life. Several smart therapeutic strategies have been developed over the years, but the 90% of them fail in the clinical phase. The main issues responsible for their unsuccessful clinical translation are strictly related to the adopted preclinical validation tools that do not accurately reproduce CW complexity. In this perspective, three dimensional (3D) in vitro wound models are promising tools to preclinically test advanced therapies/wound dressings in a more relevant and ethically sustainable scenario. In this context, this work aimed at engineering a multifunctional bioink for the fabrication of 3D cellularized constructs via microextrusion bioprinting as potential in vitro wound models.