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Translating Aptamer-Modified Nanopipettes to Complex Systems

Anna Burdina

Translating Aptamer-Modified Nanopipettes to Complex Systems.

Rel. Matteo Cocuzza. Politecnico di Torino, Corso di laurea magistrale in Nanotechnologies For Icts (Nanotecnologie Per Le Ict), 2022

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Dopamine is a type of neurotransmitter and hormone. It plays a role in many important body functions, including movement, memory, pleasurable reward, and motivation. High or low dopamine levels are associated with several mental health and neurological disorders. With these diseases on the rise, it is imperative to improve our understanding of the basics of neuronal transmission. Limitations in the selectivity and sensitivity hinder most biosensor platforms from detecting physiologically relevant changes in neurotransmitter concentrations in real-time. Moreover, spatial resolution nearing synaptic ranges 20-40 nanometers (nm) stays challenging for most of the state-of-the-art techniques. Dopamine-specific nanopipette sensor claim to be a promising candidate for serving these issues. The working mechanism of a nanopipette is a combination of ion rectification effect which allows sensing extremely small ion changes in buffer solution and/or sensor’s surface charge alternations, and surface coating with specific molecules termed aptamers that provide selectivity. This Master’s Thesis focuses on the characterization of the dopamine-specific nanopipette working principle. More precisely, the origin of the nanopipette current response was investigated by means of QCM-D and a novel technique termed Focal Molography. Several hypotheses had been put forward in order to explain diverse sensor behavior in presence of divalent cations. In addition, dopamine nanopipette sensors were used for dopamine quantification in complex media such as blood serum and neuron culture media. Moreover, yield analysis was performed and the whole sensors functionalization process was reviewed. As a result yield increase of nearly 2 times was achieved.

Relators: Matteo Cocuzza
Academic year: 2022/23
Publication type: Electronic
Number of Pages: 75
Corso di laurea: Corso di laurea magistrale in Nanotechnologies For Icts (Nanotecnologie Per Le Ict)
Classe di laurea: New organization > Master science > LM-29 - ELECTRONIC ENGINEERING
Ente in cotutela: ETH Zurich (SVIZZERA)
Aziende collaboratrici: ETH Zurich
URI: http://webthesis.biblio.polito.it/id/eprint/24791
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