Cardiovascular disease (CVDs) are the major cause of morbidity and mortality worldwide. Among these pathologies, myocardial infarction (MI) is responsible for 85% of the deaths concerning CVDs, leading to a significant loss of contractile cardiomyocytes and tissue remodelling with the formation of a fibrotic scar. The release of specific microRNA (miRNAs) has arisen considerable interest as a new strategy for myocardial regeneration, inducing cardiomyocytes proliferation or transdifferentiation of cardiac fibroblasts into induced cardiomyocytes (iCMs). However, the delivery of naked miRNAs has different drawbacks associated with low retention at the targeted site and possible off-target effects. Injectable hydrogels have been proposed as a promising alternative for the controlled and local in situ release of encapsulated miRNAs-loaded nanoparticles.