The purpose of this paper is to investigate innovative business models and new potential actors for the port and logistics industries created in collaboration with 5G-LOGINNOV, a European initiative started in September 2020 and developed within the ICE-ICT for City Logistics and Enterprises laboratory. This project aims to analyze 5G-enabled applications and innovative solutions that will be tested in three living labs to create a beneficial impact for the port ecosystem; it follows a stakeholder-led approach to ensure that proposed innovations are co-created with the 5G consortium partners and ports (Athens (GR), Hamburg (DE) and Koper (SLO)). Regarding the thesis structure, the preliminary part deals with the current state of the art of 5G, with an in-depth study on critical success factors (KSF) and key performance indicators (KPI), taking into account the network requirements and use cases in line with existing standards (ITU, 3GPP, ETSI).