In the recent years, there is a constant increasing need to reproduce with high fidelity the environment of cells, which is crucial for different reasons: modelling a disease in order to test a new drug, better understanding cells’ behaviour and response to certain stimuli, creating devices that can mimic our tissues or organs to avoid animal testing, and so on. This is why the focus on the production of substrates with natural and biocompatible materials is increasing really fast and why this thesis was focused on the creation of a bio-resist with biomimetic features in order to print, via two-photon polymerisation, different substrates which can be used in future works for several biomedical purposes.