The work done in this thesis shows the methodology for evaluating the Levelized Cost of Hydrogen (LCOH) starting from production till delivery at Italian Hydrogen Refueling Stations (HRS), by means of PEM (Proton Exchange Membrane or Polymer Electrolyte Membrane) electrolysers. The energy exploited for electrolysis is wind energy (i.e. a renewable source), so it ensures the production of hydrogen actually green, differently from conventional production categories such as steam methane reforming and coal or biomass gasification. It was decided to size the study by assuming a national demand for hydrogen needed to meet a market penetration of 1% hydrogen cars. Respected sources have estimated that Italy will reach 1% of hydrogen powered vehicles shortly after 20301,2 Two macro-scenarios have been analysed: a first scenario of Argentinian prpduction, in Patagonia region, where a huge wind potential at low-cost can be exploited3,4, and a second production scenario directly in Italy, saving of ocean transport expenses but with a higher cost for wind production energy.