This Master’s Degree thesis is developed within the evaluation of project economic sustainability, with a focus on “slow” mobility and specifically cycle infrastructures. The main objective was to figure a suitable evaluation tool for assessing the total cost of a cycle network over its life cycle. The most appropriate approach resulted in the Life Cycle Costing (LCC) Analysis integrated with the Cost-Benefit Analysis (CBA). The central issue in the development of a theoretical LCC guideline adapted to the field of study was the integration a of a number of cost items that are usually neglected. In fact, the application of the traditional LCC approach is generally linked to private projects and focuses mainly on owner/investor total costs, without the involvement of other stakeholders.