Tissue engineering is a branch of biomedical engineering which develops biological devices to restore and improve the functions of diseased human tissues or organs. The majority of tissue engineering techniques utilize a scaffold used to provide a suitable and native-like environment for cell attachment, growth, organization and proliferation. Scaffold’s permeability is a fundamental parameter to determine its regenerative efficiency. There are two main approaches to evaluate scaffold’s permeability: semi-empirical models which use the scaffold’s topological features to obtain its permeability, and experimental approaches based on the development of a test bench. This last kind of approach can be further divided into pump-based methods and gravity-based methods.