The aim of this thesis is to study the strategy for revitalization of damaged teeth, by eliminating infections and regenerating the dentine-pulp complex instead of traditional root canal treatment, such as the obturation of the root canal with biologically inert materials and the use of sodium hypochlorite (NaOCl), as an endodontic irrigant and antimicrobial agent. NaOCl can induce potential complications ranging from permanent bleaching of clothes to serious soft tissue damage. The main problem for a dental pulp damaged is linked to bacterial contamination of the root canals. Therefore in this work, a multifunctional nanoformulation has been developed as potential antimicrobial and differentiation agent to differentiate dental stem cells to odontoblast-like cells.The Human Cathelicidin LL37 shows important antimicrobial and pro-regenerative properties.