Cardiac tissue engineering (CTE) aims to develop functional substitutes of native myocardium to be exploited as in vitro models for cardiac development and disease research, and ultimately for cardiac repair. For the development of effective cardiac substitutes, alongside the cells and the architectural and biochemical support provided by scaffolds, a paramount role is played by the physical stimuli applied. In vitro, several studies demonstrated that electrical stimulation affects the rate, duration, and number of action potentials of cardiomyocytes, increasing the percentage of spontaneously beating cells, promoting cell–cell coupling and calcium handling, and directing the organization of cardiac cells. In this work, we designed, developed and tested a versatile electrical stimulator, ELETTRA, aimed to provide cardiac-like electrical impulses for CTE applications.