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Biosensors for detection of bacteria using fluorescence

Elisa Passalacqua

Biosensors for detection of bacteria using fluorescence.

Rel. Carlo Ricciardi. Politecnico di Torino, Corso di laurea magistrale in Nanotechnologies For Icts (Nanotecnologie Per Le Ict), 2019

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Bacteria detection plays an essential role in a variety of applications, from food contamination to public health. Moreover, fast and specific detection is extremely important. However, conventional methods (such as culture collection) tend to require several days. This lead to the development of alternative detection techniques such as optical biosensors. In this study, the metal enhanced fluorescence effect, due to the interaction of a metallic nanostructured surface with a proximal fluorophore leading to an increase in the intensity and stability of the optical signal, was exploited for the detection of bacteria such as E. coli. Starting from substrates formed by the metallic layer (either Au or Ag) and by a spacer layer (aSiC:H), the biosensor was fabricated by grafting glycans on the surface via click chemistry. Subsequently, two strains of E. coli were detected, using a sandwich strategy composed by the grafted glycans, the immobilized bacteria and fluorescent glycans deposited afterward.

Relators: Carlo Ricciardi
Academic year: 2019/20
Publication type: Electronic
Number of Pages: 27
Corso di laurea: Corso di laurea magistrale in Nanotechnologies For Icts (Nanotecnologie Per Le Ict)
Classe di laurea: New organization > Master science > LM-29 - ELECTRONIC ENGINEERING
Ente in cotutela: Université de Paris 7- Denis Diderot (FRANCIA)
Aziende collaboratrici: UNSPECIFIED
URI: http://webthesis.biblio.polito.it/id/eprint/12604
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