The present work focuses on the development of an open-air, passive microfluidic device to be employed for the detection of antigens, with the ultimate goal of performing the analysis and quantification of proteins contained within human skin oil. In the prototyping step, bovine serum albumin proteins (BSA) are detected by implementing a bicinchoninic acid (BCA) assay. The design of the device allows pumpless transport of a solution, made of BSA and DI water, and the coverage of a detection zone. The latter zone is silane-functionalized, so that proteins can be immobilized on its surface. Different wettability-patterned designs are considered and tested.