Epygon valve is the first biological transcatheter mitral prosthesis which can restore the natural blood flow vortex in the left ventricle, optimizing the ejec- tion function.The aim of the study is to in vitro investigate the fluid dynamic that develops inside a left ventricle (LV) phantom in presence of the Epygon prosthesis valve in the mitral position through two-dimentional Particle Im- age Velocimetry (PIV). In particular, the valve is tested in an anatomical and in an non anatomical orientation (180° valve rotation). To this purpose, a commercially-available cardiovascular simulator (Vivitro PulseDuplicator) was employed to simulate the left heart and to reproduce working conditions ac- cording to the ISO 5840-2021 standard.