The constant exposure to toxic substances in the environment may be correlated to the increase in cardiac pathological conditions, such as cardiac fibrosis or cardiovascular diseases (CVDs), that usually affect the elderly population. Nowadays there is a strong pre-clinical research’s dependence on animal models or 2D human in-vitro models, both of which present significant differences from human real-case scenarios. A new approach is represented by 3D in-vitro models that allow for the use of human cells, higher throughput, tunability of cell composition and tridimensional environment. In collaboration with H2020 Project ALTERNATIVE, the aim of the work is the development of a cardiotoxicity screening platform of industrial chemicals and pharmaceuticals, focusing most of all on the effects on the elderly population.