Valve prostheses are obtained through a design process involving in-vitro behavioural analysis with pulse duplicators that mimic the human heart and cardiac cycle. The pulse duplication device therefore plays an essential role during the in vitro analysis of a valve, which requires careful design. This last aspect is the focus of this thesis, in which supporting analyses were carried out for the creation of a new pulse duplicator model (currently under development at the Tissue Engineering Laboratory of the Rimed Foundation), which will be used to test a new mitral valve model in vitro. The customised pulse duplicator model aims to replicate left-sided chambers of the heart, consisting of atrial and ventricular chambers and offers the possibility to accommodate both the mitral valve (the main object of investigation) and the aortic valve.