Gene delivery holds immense promise in advancing therapeutic interventions by enabling precise regulation of cellular functions. This study aims to characterize the complexes formed by polymeric nanoparticles encapsulated in extracellular vesicles (EVs) and assess their potential for targeted gene delivery to dendritic cells (DCs). DCs, as professional antigen-presenting cells, play a crucial role in initiating and modulating immune responses, making them attractive targets for therapeutic interventions. The focus of this work lies in the comprehensive characterization of polymeric nanoparticle-encapsulated EV complexes, including their shape, dimensions, toxicity, and encapsulation efficacy. By assessing these crucial parameters, we gain insights into the physical properties and safety profile of the complexes, which are essential for successful translation into clinical applications.