Vehicle-to-Grid (V2G) is a technology that enables plug-in vehicles to supply energy to the grid in order to provide ancillary services to it (like secondary frequency reserve). The Italian TSO (Transmission System Operator) Terna uses a dedicated market called MSD (Dispatching Services Market) to purchase the services that guarantee continuity and stability of the power supply. In the last few years, Terna began pilot projects for increasing the number of suppliers that can provide ancillary services. One of these is the UVAM (Virtually Aggregated Hybrid Units) project, that includes among the different assets also electric vehicles. This thesis aims to show that the economic convenience exists if V2G technology takes part in Secondary Frequency Regulation.