Carbon Dots (CDs) are extremely promising fluorescent nanoparticles. These particles are frequently used in Nanomedicine as biosensors, drug carriers and imaging probes. In this project CDs are synthesized to interact with nucleic acids to promote gene delivery and therapy. Is convenient to use nanoparticles as DNA/RNA vectors because they can be better engineered, tuned and it’s possible to control collateral effects. Folic Acid, Citric Acid, o-Phenylenediamine and Polyethylenimine 25 kDa were used as precursors. After a purification step, the particles were characterized in term of: UV-Vis spectrum, Fluorescence spectrum, AFM images, TEM images, Zeta Potential, TGA, FTIR and Mass spectrum. Following these preliminary characterizations, made to evaluate features and chemical structure of the particles, DNA affinity tests were performed.