The Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) is an ultrasensitive mechanical sensing device capable of providing real-time, non-invasive measurements of changes in resonance frequency and energy dissipation responses due to cells immobilization onto the sensor surface. Most of its applications in cell research have been limited to the study of adhesive interaction between cells and the substrate surface and the evaluation of the effect of external stimuli on the adherent cells. The aim of this thesis work was to further exploit the capabilities of the QCM-D to study cell adhesion on different substrates. Firstly, it was introduced the mechanism of cell adhesion on the QCM-D sensor surface characterized by a three-phase response profile: cells adsorption, attachment and spreading, and remodelling.