Most bacteria can adhere to various surfaces, included human tissues, developing a complex 3D structure called biofilm[5], which has high resistance to disinfectant chemicals and antibiotics[6].Commonly in hospitals, the analysis of antibiotics resistance is carried out using static bacterial cultures in Petri dishes, which are time and resource consuming and then they do not mimic the in vivo like conditions.In the last few decades in research laboratories, bacterial cultures are also carried out in microfluidic systems where bacterias are cultured in flow fluids mimicking, in this way, more in vivo like conditions. However, most of the pumps used to move liquid in the microfluidic systems are complex and bulky, often requiring large amount of reagents and culture medium[7].Centrifugal microfluidic platforms, “also referred to as lab-on-a-disc (LOD)”, have a simple fluid actuation module to pump and handle the fluid inside the platforms.