This thesis explores immunomodulation as a pivotal approach to skeletal regeneration, with macrophage polarization identified as a key regulator of healing outcomes. The work focuses on the combined use of low-intensity pulsed ultrasound (LIPUS) and microfluidic-assisted 3D bioprinting. LIPUS offers a noninvasive strategy to modulate cellular activity and resolve inflammation, while microfluidic bioprinting enables the precise spatial organization of bioactive signals and cell-laden architectures.