Skeletal muscle tissue engineering aims to develop functional muscle constructs in vitro for applications in regenerative medicine, drug testing, and in vitro disease modeling. Efficient maturation of engineered muscles relies on the precise recapitulation of the native biophysical stimuli. In vivo, electrical pulses are essential for coordinated contraction of the motor units. In vitro, the ability to deliver defined patterns of stimulation is essential to induce a contractile behaviour in the cultured constructs, promote cell differentiation, and replicate a physiologic-like activation, in the form of single twitches or of tetanic contractions. In this thesis, a novel electrical stimulation system for skeletal muscle tissue engineering was developed and tested.