Gelatine methacryloyl (GelMA)-based hydrogels are emerging as innovative and promising 3D platforms, increasingly employed in many fields of biomedical research due to their high versatility, biocompatibility and tunability of mechanical properties. Derived from gelatine and functionalized with methacrylic anhydride, GelMA can be precisely tailored by adjusting set up synthesis, enabling the formation of highly customizable 3D matrices via photo-polymerization. This study aimed to assess the potential of GelMA-based hydrogel as a support for mimicking the extracellular matrix (ECM) of metastatic colorectal cancer (mCRC), the third most frequently diagnosed malignant tumour worldwide. Nowadays 3D in-vitro models are widely adopted to investigate the biological processes underlying pathologies as mCRC, and as platforms for the evaluation of new therapeutic agents, due to their ability to faithfully reproduce the 3D native tissue architecture.