The Epygon valve (Affluent Medical) is the first ‘physiological’ biological transcatheter mitral prosthesis specifically designed to restore the natural blood flow vortex in the left ventricle. Its asymmetric design, characterized by a pericardial tissue mono-leaflet combined with a D-shaped annular ring, aims to accommodate annulus dynamics and ensure physiological ventricular filling and ejection. The objective of this study is to investigate the intraventricular fluid dynamics induced by the presence of the Epygon valve in mitral position, through two-dimensional Particle Image Velocimetry (2D PIV), by observing the flow fields under two cardiac output conditions: 3.5 L/min and 5 L/min. To this purpose, the ViVitro Pulse Duplicator system, equipped with a silicon ventricles and transparent chambers, was employed to simulate the left heart circulation, reproducing working conditions in compliance with UNI EN ISO 5840:2021 — namely, a heart rate of 70 bpm, a mean aortic pressure of 100 mmHg and a systolic duration of 35% of the cardiac cycle.