Reactive Oxygen Species (ROS) are present at the intracellular level and play a crucial role in metabolic regulation, influencing key cellular processes. However, if ROS levels become excessive, as a result of an uncontrolled dysregulation, they lead to oxidative stress within cells and tissues. Oxidative stress is involved in the onset and progression of many pathological conditions, including cardiovascular diseases, which are one of the leading causes of mortality worldwide. Among the strategies to manage oxidative stress, chitosan is known to act as ROS scavenger. However, major challenges remain in the context of in vitro reproduction and modelisation of in vivo oxidative stress conditions.