To analyze the progression of a disease, the response to a treatment, or the effects of drugs, it is essential to study the dynamic behavior of cells and the morphological changes they may undergo over time. For this reason, the analysis of microscopy videos is a fundamental practice in biology and medicine. This thesis work has been developed in collaboration with the REAP project, an initiative of nine multidisciplinary European teams dedicated to developing advanced imaging tools for studying Drug Tolerant Persister cells (DTP) in breast cancer. The most common type of cancer  worldwide and the primary cause of cancer-related deaths in women is breast cancer.