Ultrasound (US) imaging represents the most used technique for assessing pennate muscle architecture and its functionality. Fascicle tracking is a method that provides information about changes in architecture during muscle contractions. It consists of continuously tracking one or more fascicles within a target muscle throughout a sequence of US images. The main aim is to follow the fascicle position and orientation during passive or active movements. To estimate this information, fascicle length (FL), and pennation angle (PA) changes are typically computed along the analyzed US sequence. Fascicle tracking potentialities cover many fields, such as rehabilitation, medicine and sport science. Various fascicle tracking algorithms have been presented in literature, to offer an alternative to time-consuming and subjective manual tracking.