Microtubules (MT) are small cellular components that play a fundamental role in development, proliferation, spreading, and signaling of cells. The basic lattice of MTs is composed by the alternation of α and β protein subunits called tubulins. Over the decades, they have become the main target of drugs involved in the treatment against tumors. Chemotherapy drugs can be divided into two main categories: stabilizing and destabilizing agents, which, according to their properties, can stabilize or destabilize the lattice of MTs, leading to cell apoptosis. This is the case of Paclitaxel (PTX), a prominent chemotherapy drug used in breast cancer treatment. It aims to stabilize MTs through the firming of longitudinal and lateral contacts.