Multispectral optoacoustic tomography (MSOT) is an emerging imaging technique that combines the advantages of both optical and ultrasound imaging to achieve high-resolution functional imaging. It offers several key advantages for imaging biological tissues and organisms, making it a promising tool for various applications. It’s based on the photoacoustic effect that is induced when a nanosecond laser pulse illuminates the tissue. The light energy at specific wavelengths is absorbed by chromophores, that can be endogenous (like haemoglobin, lipids, etc) or exogenous, which leads to a thermoelastic expansion resulting in the generation of ultrasound waves. These waves are detected by the probe and can then be processed by an elaboration system in order to obtain the raw signal, known as synogram.