This work presents the development of a hardware-in-the-loop (HiL) test bench that can be leveraged to validate an e-bike anti-lock braking system (ABS) for multiple bicycle geometry, loading and test scenarios. The approach consists of interfacing a virtual bike simulation model running on a real-time target machine with the physical ABS hardware under test. This setup allows the test engineer to first test and evaluate the ABS behaviour in a safe place, before starting tests on the track. This thesis describes the derivation of a six-degrees-of-freedom (dof) equation-based model representing the longitudinal in-plane dynamics of an e-bike. The simulation model is experimentally validated against measurements conducted on an instrumented test bike.