Advanced therapies are developed every day to find alternative procedures to life-threatening diseases. In the field of cancer research, tissue-engineered 3D cancer models have been developed to achieve more accurate biomedical representations. Although state-of-the-art 3D models still lack in the modelling of the complexity of the native tissue, they represent a first step towards reaching the goal in terms of the 3R (replacement, reduction, and refinement) framework. In the present Master Thesis, the purpose is to develop two systems enabling the study of a 3D model of colorectal cancer (CRC) to evaluate the evolution of the tumour and eventually the efficacy of a combined treatment of acoustic external stimuli and biomimetic stimuli-responsive hybrid nanoparticles.