In vitro models of epithelia have been frequently obtained by air-liquid interface (ALI) culture on commercially available transwell plates. Transwell inserts are standardized, have high costs and restrains regarding choice of materials. Based on that, the thesis was aimed at designing an in vitro model of esophageal epithelium by in vitro air-liquid interface (ALI) based on cellularized hydrogel, casted in a custom 3D printed inserts with tailorable dimensions to fit into different sizes of well plates and grid porosity to use different cell culture strategies. The inserts were fabricated using fused deposition modeling (FDM) from the biocompatible polymer polycaprolactone (PCL). The hydrogel matrix was based on gelatin methacryloyl (GelMA), a biocompatible derivative of gelatin.