Antibiotics are extensively used for human and animal treatment and their global consumption is estimated will increase. Due to their low biodegradability, they cause serious environmental pollution. The phenomenon of antibiotic resistance is the main risk to human health because the microbial community, thanks to evolution processes, can survive common antibiotics, making their action ineffective. Thus, the goal of this study is to identify fungal laccases that are active on antibiotics and to perform a feasibility study for the development of immobilized-enzyme bioreactors. Firstly, it was measured the reduction of bactericidal activity of six after treatment with four different laccases derived from five different sources, showing the inactivation of amoxicillin and tetracycline by commercial laccase from Aspergiullus sp.