In the last couple of decades, interest towards the cultivation and usage of microalgae has grown, thanks to their possible application in the food and the cosmetics industries, in wastewater treatment, in biofuel production, and for CO2 fixation. However, despite their great range of applications and their advantages (like a limited footprint), microalgae cultivation at industrial level is held back by some factors, such as the large water volume required for the biomass growth and the relatively high costs that characterize the process of harvesting and dewatering. The aim of this study is to investigate the dewatering of Scenedesmus Obliquus through 0.14 µm ceramic membrane filtration, analyzing the regulating factors and the possibility of using the obtained permeate as a medium for the growth of a new culture.