Exosomes Extracellular Vesicles (EEVs) have acquired great interest in biomedical field, due to their biological contents (such as proteins, mRNAs, miRNAs), which can make them potential biomarkers for early detection, diagnosis and treatment of cancer and other diseases. However, exosome isolation and detection for biomarkers analysis are challenging. In fact, the standard methods for exosomes analysis are time-consuming, costly, and require big laboratory instruments, so, the theranostic potential of exosomes is difficult to exploit. In the last decade, microfluidic platforms for exosome analysis have been developed, because at this dimensional scale it is possible to manipulate small amounts of sample volumes, to conduct separations and detections with high resolution, to reduce the laboratory analysis costs and, also, the time requested for the analysis.