Microtubules (MT) play key roles in cell mitosis and in particular they help to segregate chromosomes. For this reason, MT are a desirable target in cancer treatment for the inhibition of the unregulated tumoral cells division. In addition, several tubulin isotypes exist, each one having its own sensitivity to a specific drug and a different level of expression depending on tissues. This represents an important opportunity in drug design, which exploits the isotypes expression to study and synthesize highly specific drugs, having a greater affinity only for diseased cells and causing reduced systemic effects. Antimitotic drugs act by interfering with MT polymerization-depolymerization kinetics, but the molecular mechanisms responsible for the drug activity is still unclear.