This master thesis project focuses on the realization of an advanced in-vitro model of Blood-Brain-Barrier (BBB). The model innovation is the possibility to test drugs and nanovectors permeability, understanding which therapies could be effective in brain cancer treatment, and in particularly for glioblastoma multiforme. The aim of this model is to overcome all the limitations related to ineffective 2D and animal models, the latter usually related also to ethical concerns. The polymeric porous and biocompatible membranes were fabricated, via vapour induced phase separation (VIPS). These supports were mechanically and surface characterized, and their morphological images were taken by FEGSEM microscope. These chemical and physical procedures were performed at the Materials Engineering Department of Loughborough University (UK).