Thermoplastic polyurethane elastomers (TPUs) are appealing to the industry world because they combine a broad range of properties and ease of production. By introducing supramolecular moieties in the polymer architecture, a dynamic reversible network is formed and self-healing ability is imparted to the material. This is undoubtedly beneficial since it extends the lifetime of components and consequently reduces resources consumption. Despite many reports of efficiently healing polyurethanes, no research has focused on understanding the relationship between interfacial healing and TPUs microstructure. To this end we synthesized by one-shot method a set of segmented polyurethanes by reacting a highly branched polyol, 2-ethylhexane-1,3-diol (EHD) and three commercially available diisocyanates.