Microtubules (MTs) are involved in several cellular processes and play a key role during mitosis, forming the mitotic spindle which is able to divide the chromosomes. The connection between microtubules and chromosomes is guaranteed by a macro structure including various motor proteins, called kinetochore. Microtubule instability, characterized by lengthening and shortening of the MTs, generates forces at the kinetochore, that lead the chromosomes toward the metaphase plate. Therefore, the stability of the junctions between kinetochore and plus end of the microtubule is a critical issue for an accurate chromosome segregation. The stability of the kinetochore-microtubule interaction is guaranteed by a W-shaped homodimer of coiled coils, called ska complex, which is crucial for a correct cell division in human cells: the MT-binding domains (MTBDs) of the ska complex recognizes tubulin monomers making transversal bindings.