Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal tumor affecting the exocrine pancreas. Its poor survival rate is attributed to late-stage symptom onset, invasive metastasis, and limited therapeutic options. To address these challenges, in vitro models that replicate the pancreatic tissue microenvironment are crucial for therapy development and to predict drug efficacy. Organ-on-chip technology offers a powerful approach to achieving these goals. In this thesis project, a microfluidic device is developed to model the cellular composition of the pancreatic acino-ductal unit. The device comprises a multilayer structure using Polydimethylsiloxane (PDMS), with three layers, two of them separated by a membrane. In particular, the bottom layer is loaded with human foreskin fibroblasts (HFF-1) embedded in a type I collagen hydrogel to mimic the stromal component, while the top layer reproduces the healthy or cancer epithelial component, using human pancreatic ductal epithelial cells (HPDE).