The human body possesses a large variety of barriers, of different origins and characteristics, playing an important role in the functioning of the organism. They are responsible for controlling some essential biological processes and maintaining homeostasis by regulating the interactions between the compartments that they separate. A possible disruption of the barrier can lead to important consequences. It can indeed be associated with severe diseases including multiple sclerosis in the case of disruption of the blood-brain barrier or disorders such as celiac disease in the case of loss of intestinal barrier integrity. Monitoring the status and integrity of a cell barrier is therefore crucial.