In the last decade, biomedical research has been mainly dedicated to the development of new microfluidic devices that can be used in medical, biological, and pharmacological applications. These miniaturized systems contain micrometer chambers and channels for the flow or containment of fluids, therefore excellent for the fabrication of 3D lab-on-chip for cell cultures and biological analysis. In this regard, it was necessary to promote new large-scale production technologies that were profitable in terms of cost and time. The techniques of 3D Printing, or Additive Manufacturing, are an advantageous alternative to traditional manufacturing techniques of microfluidic platforms. All this is allowed by its promising properties, such as high precision, the reduction of production waste, the possibility of manufacturing complex components in a short time.