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Induction heating based on thermal lysis system development for a diagnostic consumable

Francesca Sansipersico

Induction heating based on thermal lysis system development for a diagnostic consumable.

Rel. Matteo Cocuzza, Ivan Ferrante. Politecnico di Torino, Corso di laurea magistrale in Ingegneria Elettronica (Electronic Engineering), 2021


Sepsis is a life-threatening organ dysfunction caused by a disregulated host response to infection. It is the main worlwide cause of death because of it's hard to be recognized. Every year in USA 270 000 adults, out of 1.7 million contracting this desease, die. Normally, the triage for the identification of sepsis is based on SOFA. Unfortunately, this method does not ensure the diagnosis and, hence, the recovery from sepsis. A new method for a fast diagnosis is required. PCR has been chosen to be accurate and rapid because it allows to detect the presence pathogens in a blood sample. Mobidiag is an international company that develops solutions for infectious deseases and antibiotic resistance. In this scenario Novodiag is placed: it is an innovative platform that combines PCR and microarray technologies. The patient's sample is placed in a consumable tool, cartridge, that performs the analysis in approximately one hour with high accuracy. For the Sepsis project, Novodiag has been updated adding the possibility to perform the lysis in the consumable, through induction heating. Lysis is the mechanism that allows to disrupt the cell membrane in order to extract the DNA of a pathogen. The main goal of this thesis is to validate the concept of induction heating and the system optimization in a biological context. The study has been performed in an experimentally way and supported by Comsol simulations. The induction heating system consists in:a ZVS module, a pancake coil and a reaction chamber . They are managed by a five-minute heating protocol running on Arduino. Each of these components has been studied to boost up the performance. Necessary step for the system optimization has been to uniformly heat the workpiece. To achieve that, ZVS module has been studied and improved considering the frequency and the power that it supplies to the coil. Consequently, its frequency has been increases to 230kHz changing the inductance, enhancing the energy transfer between coil and workpiece as well. Moreover, the advantage of high frequency is to reduce the heating dissipation of the coil, due to Joule effect. In addition, thanks to COMSOL simulation, the magnetic field of a pancake coil has been simulated varying geometrical parameters, such as the thickness of coil's wire, the spacing between them and the number of coil turns. Then, the performance of the lysis has been investigated using this optimized induction heating system. Arduino allows to reach temperatures in between 120-130°C. This range is effective in lysis of yeast (S. cereviasiae).

Relators: Matteo Cocuzza, Ivan Ferrante
Academic year: 2021/22
Publication type: Electronic
Number of Pages: 66
Additional Information: Tesi secretata. Fulltext non presente
Corso di laurea: Corso di laurea magistrale in Ingegneria Elettronica (Electronic Engineering)
Classe di laurea: New organization > Master science > LM-29 - ELECTRONIC ENGINEERING
Ente in cotutela: Genwave Mobidiag France (FRANCIA)
Aziende collaboratrici: Genewave Mobidiag France
URI: http://webthesis.biblio.polito.it/id/eprint/20425
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