Tissue engineering (TE) aims at generating functional tissue constructs to be used for repairing or replacing damaged human tissues or as in vitro tissue models for investigating the physiological/pathological tissue mechanisms. Biological constructs consist of two main elements: substrate and cells. Commonly, monolayer static cell cultures are performed by using flat rigid substrates. However, for promoting the formation of functional constructs, dynamic culture conditions on flexible substrates would be more appropriate in some cases. In the last decades, bioreactors have become crucial tools in TE research. Indeed, they allow for mimicking the native dynamic environment by providing specific physico-chemical stimuli in vitro.