The process of developing and bringing into market new drugs represents one of the fundamental activities which improves human health and quality of life. The preclinical phase of the Drug Development Process is considered a crucial step for the discovery of new potential pharmaceutical compounds. In fact, at this stage, screening of new molecules and essential pre-tests are carried out using in vitro models. In this view, in vitro systems are valuable tools to understand cell mechanisms and the effects of drugs on a culture. A wide range of in vitro models have been created during the years, where the majority are based on static 2D cultures.