Cancer is the second leading cause of death in industrialized countries. Tumors affect different organs and tissues; they are characterized by uncontrolled cells proliferation which impair function and structure of involved body area. The high mortality rate of cancer is due to generation of metastases, which seems to take place only in specific organs, mainly the liver. Mechanical properties of extracellular matrix are known to support cell survival and spreading and might therefore play a role in the metastatic spreading of cancer. Here, we aim to prepare different substrates of similar fibrous morphology but different mechanical properties that mimic those of organs most affected by metastasis: liver, bone and lung.