The development of functional cardiac in vitro models is essential to improve drug screening and to understand fundamental cardiac regeneration mechanisms. For the development of Engineered Cardiac Tissues are essential the presence of cells housed in scaffolds, which provide the architectural and biochemical support, but a paramount role is played by the physical stimuli applied. Electrical stimulation is one of the key physical stimuli capable to greatly support cardiac maturation and functionality. In the heart, the electrical stimuli are useful for generating impulses that allow the coordinated contraction of cells. In vitro, several studies demonstrated that electrical stimulation influences the rate and duration of action potentials of cardiomyocytes, increasing the percentage of beating cells, directing the organization of cardiac cells and promoting cell-cell coupling and calcium handling.