This work describes the Master’s Thesis project carried out at the University of Massachusetts, Amherst. The goal of this project is to create selective and effective bioorthogonal nanocatalysts for the treatment of biofilm-associated infections. The antimicrobial activity through the activation of a pro-drug using polymeric nanoparticles encapsulating hydrophobic transmission metal catalysts (TMCs) is analysed. Bioorthogonal chemistry has emerged as a promising strategy for modulating bioprocesses through reactions that cannot be achieved by natural enzymes and TMC-mediated bioorthogonal catalysis has not been previously demonstrated for the eradication of biofilms. Based on a previous work [1], the quaternary ammonium polymer (PONI-C11-TMA) is used to synthesize polymeric nanoparticles (PNPs) encapsulating iron-porphyrin [Fe(TPP)]Cl and the characterization of dimension and catalytic behaviour, performed by the in vitro activation of a resorufin-based pro-fluorophore, are carried out.