Tissue engineering is an approach of regenerative medicine aimed at the integration, proliferation and maturation of isolated cells in an artificial environment, often based on biocompatible materials, with the final goal of repairing or substituting a damaged organ or tissue. Among these materials, micro and nano-structured scaffolds with physical and chemical properties tunable on-demand by an external source of stimulation, also known as “smart materials”, represent promising tools for favouring a particular cellular behaviour, for instance during both proliferation and differentiation events. This Master Thesis work is focused on the fabrication and characterization of nanostructured zinc oxide (ZnO) smart piezoelectric scaffolds for bone tissue engineering that can be remotely activated by means of a mechanical stimulation provided under the form of ultrasounds (US), with the goal of generating physiologically relevant piezo-potentials favouring cell proliferation and differentiation.